Animals locally bred adult swissstrain albino mice of both sexes, weighing2533gwereused. Proteomic analysis of three medically important nigerian naja. Adobe acrobat dc version 15 or higher or adobe acrobat reader. Naja cytotoxicity insulinotropic activity threefinger toxins phospholipase a2 abstract four peptides with cytotoxic activity against brinbd11 rat clonal. How to split a pdf file tutoriais do adobe acrobat dc. Poster session presented at symposium for biological and life science students symbls 2016, cambridge, united kingdom.
Phospholipase a2 pla2 from naja naja atra taiwan cobra snake venom was subjected to lysine modification with trinitrobenzene sulphonic acid tnbs, and two major trinitrophenylated tnp derivatives, tnp1 and tnp2, were separated by h. The final rfactor, computed with all xray data available, is 17. Investigating the effects of naja nigricollis venom on colorectal and pancreatic cancer cell lines to identify biomarkers to aid cancer treatment iqra farooq1, steve trim2, emily knight1, carol trim1 contact information. Studies on the status of lysine residues in phospholipase. Albino rat, venom, naja nigricollis, snakebite, bovine blood, uvaria chamae. Ability of gamma radiation to detoxify blacknecked. Pdf ethnomedical treatment of poisonous snakebites. Theanimalswerefedstandard mouse pellets, and water was supplied ad. Aja manufacturers awardwinning video acquisition, conversion, io, and streaming solutions offering unparalleled flexibility, performance and reliability. Although blood coagulation requires the assembly of complexes on phospholipid membranes, the differences in anticoagulant potencies of the phospholipases.
The species is largely nocturnal, and is often found while crossing roads at night. Pdf moringa oleifera is a plant that has several medicinal properties, however. In vitro and in vivo neutralizing activity of uvaria. Tnp1 contained only one tnp group on lys6 and showed a marked decrease in enzymic activity, but still retained 45% of the lethal toxicity. The specific epithet nigricollis is latin for blacknecked. Full text is available as a scanned copy of the original print version. Reusing this file this work is free and may be used by anyone for any purpose. Portulaca oleraceawere room dried, powdered and extracted in ethanol. Theanimalswerefedstandard mouse pellets, and water was supplied ad libitum. Snakebite is associated with a diverse pathophysiology due to the magnitude of variation in venom composition that is observed worldwide.
In vitro and in vivo neutralizing activity of uvaria chamae. The effect of extract was determined by pretreating a naja nigricollis west african black spitting cobra were group of mice n. There is a folkloric claim that uvaria chamae is beneficial for the management of snakebite and wounds in african ethnobotanical surveys. Kompres atau optimalkan file pdf secara online, dengan mudah dan gratis. Adobe systems is a software manufacturer that has created many document and multimedia editing programs. An oversized pdf file can be hard to send through email and may not upload onto certain file managers. Uses html5 to display pdf files directly in the browser. Their coloration and markings can vary considerably. Download our free pdfcreator and start converting your pdf files. Making a pdf file of a logo is surprisingly easy and is essential for most web designers. Six cobra species are listed on the live import list. This file is licensed under the creative commons attributionshare alike 3.
How to shrink a pdf file that is too large techwalla. Portable filebased recorder and player aja video systems. Publishers pdf, also known as version of record link back to dtu orbit citation apa. This article explains what pdfs are, how to open one, all the different ways. Results revealed a total of 57, 55, and 46 proteins in the venoms of n. Isolation and characterization of cytotoxic and insulin. Spitting cobra, black necked spitting cobra turkce. It was first described by norwegian zoologist johan reinhardt in 1843. Lethality of naja nigricollis reinhardt venom and antivenom.
Pathogenesis of dermonecrosis induced by venom of the. In vitro and in vivo neutralizing activity of uvaria chamae leaves fractions on the venom of naja nigricollis in albino rat and bovine blood volume. In this work, the pla2 was purified by two steps of fractionation. Anti snake venom potential of securidaca longepedunculata. Proteomics technologies enable a comprehensive study of complex proteins and their functions.
A pdf portable document format is a widely popular type of document format created by adobe. Actions of whole and fractionated indian cobra naja naja. Pdf is a hugely popular format for documents simply because it is independent of the hardware or application used to create that file. Freshly collected uvaria chamae leaves were air dried, powdered and extracted in methanol. Pdf uvaria chamae annonaceae plant extract neutralizes. Luckily, there are lots of free and paid tools that can compress a pdf file in just a few easy steps. This highly flexible pdf converter is easy to use and aligns with your requirements. Rats envenomed with ld 50 of snake venoms displayed complete protection of naja nigricollis treated rats only with methanol extract and dcm fraction in 48h, and of bitis arietansenvenomed animals with all tested doses of methanol extract at 24h and the aqueous fraction in 48h. The crystal structure of toxin gamma from naja nigricollis has been solved and refined to 1. As for the biochemical responses of naja nigricollis venom, the effect of half ld 50 of native or irradiated 1. This study investigated the in vitro neutralizing effects of mucuna pruriens seed and mimosa pudica root extracts on venoms of naja nigricollis and bitis arietans. Department of veterinary pharmacology and toxicology, ahmadu bello university, zaria. This file is licensed under the creative commons attribution 2. They are moderately sized snakes that can grow to a length of 1.
Pdf a new species of spitting cobra naja from north. Pdf impact of naja nigricollis venom on the production of. Response of pc bxpc3 cells to varying concentrations of na. Information from its description page there is shown below. Pulsus group medical research journals open access journals. Navigation dashboard any live stream write permission level any 1. Naja nigricollis woodi black spitting cobra southern namibia, namaqualand northern cape to citrusdal western cape food eats lizards, other snakes, amphibians, small fish, birds, bird eggs and rodents. Studies on the status of lysine residues in phospholipase a2. Cpk, ckmb, ldh and ast after 1, 2, 4, 24 hours of envenomation.
Duplicate doiidentification number to impact of naja nigricollis venom on the production of methaemoglobin. Blood clotting time was measured using the modified method of igboechi and anuforo and bleeding time was. Naja nivea cape cobra naja nigricollis spitting cobra naja annulifera snouted cobra naja melanoleuca forest cobra 23. The pdf format allows you to create documents in countless applications and share them with others for viewing. Blacknecked spitting cobra naja nigricollis blacknecked spitting cobra naja nigricollis courtesy of gerhardt nieuwoudt, south africa. Sep 01, 2016 the pathogenesis of dermonecrosis induced by naja nigricollis venom was studied venom induces edema, blistering and necrosis of dermis and epidermis. Effect of methanol leaf extract of costus afer with naja. May 01, 2018 a file drop down menu on the aja miniconfig application bar allows you to save the current state of the miniconverter to a preset file for later recall. Investigating the effects of naja nigricollis venom on. If you wish to use this content, you do not need to request permission as long as you follow any licensing requirements mentioned on this page. The venom proteomes of three medically important nigerian elapidae snakes naja haje, naja katiensis and naja nigricollis was studied using hilic coupled with lcmsms analysis. Vcl component suite to edit, merge, split, overlay, stamp, view, print, encrypt, bookmark, and annotate pdf documents.
Antidote potential of ethanolic leaf extract of portulaca. Pdf file or convert a pdf file to docx, jpg, or other file format. Snakebite envenomation is an affliction currently estimated to be killing upwards of 100,000 people annually. Naja is a genus of venomous elapid snakes known as cobras true cobras. Ki pro combines the power of a file based recorder with the familiar controls. Cytotoxins of the threefinger toxin family are the main responsible of the effect. The rcsb pdb also provides a variety of tools and resources. Stonefish antivenom equine australian antivenom synanceia trachynis stonefish also may be effective against scorpion fish and lion fish. Pdf antivenom activity of moringa oleifera leave against.
This work is also an attempt to present a list of animals use for. The two neutral isoenzymes, cmi and cmii, are weakly anticoagulant, whereas the basic enzyme, cmiv, is strongly anticoagulant 23. Naja haje banded egyptian cobra, naja melanoleuca forest cobra, naja naja indian cobra, naja nigricollis blacknecked spitting cobra, naja nivea cape cobra and ophiophagus hannah king cobra. Depending on the type of scanner you have, you might only be able to scan one page of a document at a time. Members of the genus naja are the most widespread and the most widely recognized as true cobras. Various species occur in regions throughout africa, southwest asia, south asia, and southeast asia. The anti snake venom activity revealed that root bark extract are able to neutralize snake venom at 300mgkg body weight with 100% survival. The phylogeny of cobras inferred from mitochondrial dna.
An nmr study of the interaction of cardiotoxin y from naja nigricollis with perdeuterated dodecylphosphocholine micelles marc dauplais, jean michel neuma, suzanne pinkasfeld, andrb menez and christian roumestand dkpartement dlngknierie et detudes des prodines, cea saclay, gifsuryvette, france. Lyophilized naja nigricollis venom was purchased from the department of pharmacognosy and drug development, ahmadu bello university, zaria nigeria. You may do so in any reasonable manner, but not in. A range of snake venoms induce the oxidation of hb the ability of venoms to oxidise hb into methb has been reported within the family of viperidae 15 17,26. All six species require an import permit and conditions apply to their import and keeping. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. Several other elapid species are also called cobras, such as the king cobra ophiophagus hannah and the rinkhals. This means it can be viewed across multiple devices, regardless of the underlying operating system.
The pooled venom was lyophilized, stored in an airtight container and kept for the intended research use only. Purification and characterization of phospholipase a2. Detoxifying action of aqueous extracts of mucuna pruriens seed and mimosa pudica root against venoms of naja nigricollis and bitis arietans volume. Ability of gamma radiation to detoxify blacknecked spitting. Download pdfcreator free to create pdf files, merge pdfs, secure your pdf documents and use its many automated features. Impact of naja nigricollis venom on the production of. Oken crassulaceae, a traditional antihypertensive, antiinflammatory, anti fever and anti cancer remedy is also popular in indian traditional herbal remedies for snake bite.
Naja nigricincta is a species of spitting cobra in the genus naja belonging to the family elapidae, which is native to the deserts and drier regions of southern africa. Reinhardt used was captured and housed in a wooden cage with the help of a snake charmer. Naja nigricincta bogert, 1940, was long regarded as a subspecies of n. The generic name naja is a latinisation of the sanskrit word naga, meaning cobra. Pdf impact of naja nigricollis venom on the production. The present study includes purification and characterization of a phospholipase a2 enzyme from crude naja nigricollis venom. Adobe designed the portable document format, or pdf, to be a document platform viewable on virtually any modern operating system. The threedimensional structure is characterized by a core formed by two betasheets organized in three extended loops. To combine pdf files into a single pdf document is easier than it looks. Department of veterinary pharmacology and toxicology, ahmadu bello. Once youve done it, youll be able to easily send the logos you create to clients, make them available for download, or attach them to emails in a fo. Proteomic analysis of three medically important nigerian. I paid for a pro membership specifically to enable this feature. However, the presence of considerable variation within n.
The blacknecked spitting cobra naja nigricollis is a species of spitting cobra found mostly in subsaharan africa. The venom of naja nigricollis snake was used to test the anti snake venom efficacy of the extracts, using experimental rats. As a member of the wwpdb, the rcsb pdb curates and annotates pdb data according to agreed upon standards. The management of a bite by n nigricollis requires aggressive medical and surgical treatment. Detoxifying action of aqueous extracts of mucuna pruriens. Public health information and geographic information systems gis.
Effects of the plant extracts against venoms induced lethality, edema forming and hemorrhagic activities were investigated in mice. After collection, it was duly identified by a zoologist in the department of animal and environmental biology, kebbi state university of science and technology, aliero, nigeria. Neutralization potentials of portulaca oleracea leaf extract. In this study, we evaluate its venom neutralizing properties against naja nigricollis venom in rats. Snakebite envenomation is a global priority ranked top among other neglected tropical diseases. Somatic mutation and biological activities of naja nigricollis venom.
By michelle rae uy 24 january 2020 knowing how to combine pdf files isnt reserved. Response of crc sw620 cells to varying concentrations of naja nigricollis venom. Pdf snakebite envenomation is an affliction currently estimated to be killing upwards of 100,000 people annually. This species had long been considered to be a subspecies of the blacknecked spitting cobra naja nigricollis, but morphological and genetic. Naja nigricollis is classified under the genus naja of the family elapidae.
275 520 950 25 788 818 71 358 1616 1396 1137 1211 898 312 9 1462 109 1186 227 601 1474 243 844 873 972 1393 70 288 1522 261 868 1041 756